Composite

Part:BBa_K2779050

Designed by: Ethan Agena, Rochelin Dalangin, Anna Kim, Irene Shkolnikov   Group: iGEM18_UAlberta   (2018-09-29)


APIS Device

hemABCDEF

Our full construct APIS encodes for the genes HemA- HemF, which are the enzymes required for the production of protoporphyrin IX, a tetrapyrrole compound and is an intermediate in the heme biosynthesis pathway. The composite was assembled using 4 gblocks, and have HemB, HemE and HemF coded on the complementary strand, with T7 promoters, BBa_B0034 RBS and bidirectional terminators.

T--UAlberta--PPIXPathway.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1249
    Illegal NheI site found at 2422
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1264
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1713
    Illegal NgoMIV site found at 4974
    Illegal NgoMIV site found at 5364
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 4164


[edit]
Categories
Parameters
None